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has been cited by the following article:

• TITLE: Amelotin-Mediated Crystals Assembly in Liquid-Phase and on Tooth Surface

  AUTHORS: Xiaohua Ren, Min Zhou, Yandong Mu, Kun Tian

  KEYWORDS: Amelotin, Hydroxyapatite, Enamel, Mineralization, Electronic Microscopy

  JOURNAL NAME: Journal of Materials Science and Chemical Engineering, Vol.9 No.7, July 30, 2021

  ABSTRACT: Objectives: To study the role of amelotin in enamel formation, purified recombinant human amelotin was used in a study of amelotin self assembly and its role in new HAP crstal structure formation in a laboratory setting. Methods: The full-length human amelotin gene was cloned from developing tooth buds and recombinant amelotin was expressed and purified. Morphological changes during amelotin self-assembly and the role of amelotin in promoting new minieralization structure is studied using Transmission electronic microscopy and Scanning electron microscopy. Results: The measured molecular weight of expressed human amelotin was 28 kDa that matches the predicted values. Amelotin self-assembles to form nano-spheres 10 - 30 nm in size. The amelotin self-assembly process further produces higher 3-D structure in the forms of chain-like fabric arranged in parallel and perpendicular direction. The neonatal pearl crystals were arranged side by side to form a string of small rods and small parallel rods extend from the demineralized glaze on the surface of teeth that were pre-treated with amelotin.