TITLE:
Estimation of the galanthamine using derivative spectrophotometry in bulk drug and formulation
AUTHORS:
Karan Mittal, Ramni Kaushal, Rajashree Mashru, Arti Thakkar
KEYWORDS:
alanthamine Hydrobromide (GH); Zero Order UV-Spectrophotometry; First Derivative Zero Crossing Spectrophotometry
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.3 No.4,
April
28,
2010
ABSTRACT: Two simple, rapid, accurate, precise, reliable and economical spectrophotometric methods have been proposed for the determination of galanthamine hydrobromide (GH) in bulk and pharmaceutical formulation. First method is zero order UV spectrophotometry and second is 1st derivative zero crossing spectrophotometry. The developed methods have shown best results in terms of linearity, accuracy, precision, LOD and LOQ for bulk drugs and marketed formulations. Absorbance was measured at 287 nm for zero order and 277.4 nm for first derivative. It obeyed Lambert-Beer’s law in the range of 30-80 μg mL-1. Both methods have good linearity (r2 = 0.9997) and accuracy found to be 100.5% and 101.2% for both methods respectively.