TITLE:
Sero-Epidemiological Study of Respiratory Syncytial Virus
AUTHORS:
Mami Niida, Tetsuo Nakayama, Eitaro Suzuki
KEYWORDS:
RSV, Sero-Epidemiology, Neutralizing Antibody, IgG Enzyme Immunoassay
JOURNAL NAME:
Open Journal of Pediatrics,
Vol.10 No.3,
September
25,
2020
ABSTRACT: Background: Respiratory syncytial virus (RSV) is one of the major viruses that cause respiratory
infections in all generations, not only in neonates and infants. There is a limited
number of reports on serological epidemiology of RSV subgroups A and B. Neutralizing
test (NT) antibody
reflects protective immunity but bothersome. Sero-epidemiological study should be performed using practical
NT method. Methods: Two wild-type viruses subgroups A and B, isolated in 2013, and the Long strain was used
as the challenge viruses. NT antibody with 100% inhibition of cytopathic
effect (CPE) was examined. A total of 91 serum samples obtained from 0 to 12 years subjects without RSV infection who visited our hospital with some health
problems and 121 sera obtained from healthy subjects in different age groups were
used. Serological epidemiology of subgroups A and B was investigated in this study using new NT methods. Results: 1) A simple and practical NT method was developed.
2) The NT antibody titer was lowest in × 21.70 ± 2.03 against subgroup A and 5 × 20.85 ± 1.31 against subgroup B) and increased in 3 years of age
or older, and high antibody titers were maintained during school age. 3) A slight
difference was observed in the NT antibody titers against subgroups A and Bin young
children Conclusions: A simple NT assay method was developed in the present
study. By the age of 3 years, high NT antibody titers were observed and maintained
until 12 years. The IgG (EIA) values decreased with age. No association was observed
between IgG (EIA) and NT titers.