TITLE:
Association between Polymorphisms of Glutathione S-Transferase and Progression to Cervical Cancer in Women from Burkina Faso and Mali
AUTHORS:
Teega-Wendé Clarisse Ouedraogo, Florencia Wendkuuni Djigma, Théodora Mahoukèdè Zohoncon, Boureima Idani, Abdoul Karim Ouattara, Pegdwendé Abel Sorgho, Dorcas Obiri-Yeboah, Prosper Bado, Mah Alima Esther Traore, Birama Diarra, Albert Théophane Yonli, Charlemagne Ouedraogo, Jacques Simpore
KEYWORDS:
Glutathione S-Transferase M1-Null, Cervical Cancer, Burkina Faso, Mali
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.8 No.4,
March
31,
2020
ABSTRACT: Although persistence of high-risk human papillomavirus infection is the main risk factor, Glutathione S-Transferase highly polymorphic enzyme involved in the metabolism of xenobiotics, is a good candidate gene. The objective of this study was to compare the polymorphisms of Glutathione S-Transferase M1-null in women with cancerous lesions and without lesions. This study consisted of 322 uterine cervix samples of women from Mali and Burkina Faso with Cervical Intra-epithelial Neoplasia 2 and 3, adenocarcinoma and squamous cell carcinoma and 100 women with no lesions. Human Papillomavirus genotyping was performed by Real-time multiplex Polymerase Chain Reaction. Glutathione S-Transferase gene polymorphisms were determined using conventional Polymerase Chain Reaction followed by migration on agarose gel. A statistically significant association with high relative risks of 10.77 for the development of High grade Superficial or Squamous Intra-epithelial Lesion (95% CI = 5.59 - 20.72; p