TITLE:
New Ionic Liquids with Buffering and Chelating Abilities for Enzyme Engineering
AUTHORS:
Guangnan Ou, Biyan He
KEYWORDS:
Ionic Liquid, EDTA, Buffer, Chelator, Lipase, Peroxidise, Heavy Metal
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.10 No.10,
October
23,
2019
ABSTRACT: Ionic liquids (ILs) with buffering and chelating
abilities were designed and synthesized on the basis of
ethylenediaminetetraacetic acid (EDTA) for the development of buffered
enzymatic IL systems and for enzymatic reaction in heavy metal containing
aqueous system. Transesterification activity of Candida antarctica lipase B dissolved in the
hydroxyl-functionalized IL was buffer dependent. High activity and outstanding
stability was obtained with the buffered enzymatic IL systems for the
transesterification. In heavy metal containing aqueous system, EDTA IL buffers
as Hg2+ chelators protected horseradish peroxidase (HRP) against Hg2+-induced
denaturation and precipitation. Higher pH favored the protection, while at
lower pH the protection diminished. We can conclude that the new ILs possess
both buffering and chelating abilities.