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https://doi.org/10.1196/annals.1428.031

has been cited by the following article:

• TITLE: Variability of Deltamethrin-Resistant Metarhizium anisopliae Aggressive Strains Group to a Population of the Cossid Moth from Eucalyptus nitens

  AUTHORS: Pedro Romón, Hardus Hatting, Arturo Goldarazena, Juan Carlos Iturrondobeitia

  KEYWORDS: Coryphodema tristis, Metarhizium anisopliae, Assays, Compatibility with Deltamethrin, RAPDs

  JOURNAL NAME: Open Journal of Forestry, Vol.8 No.2, April 20, 2018

  ABSTRACT: Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was used to examine the genetic variability among Metarhizium anisopliae isolates tested to the cossid moth, Coryphodema tristis. All the isolates tightly clustered into one or the other of two groups that diverged at 12%. Results suggested that certain genotypes of the fungus, that grouped together, were able to infect moth larvae while others did not. A fragment of 760 bp, which presents high homology with a host-adaptation related protein coding gene, distinguished between aggressive and non-aggressive isolates. Neither mycelial growth nor sporulation rate or presence of known virulence genes was correlated with mortality values. Some isolates, including the most aggressive isolate ARSEF2518, were compatible with deltamethrin. Deltamethrin treatment killed all the larvae after seven days whereas fungal and mixed treatments respectively reached the same mortality after 28 and 21 days.