TITLE:
Modified Green Tea Polyphenols, EGCG-S and LTP, Inhibit Endospore in Three Bacillus spp.
AUTHORS:
Bushra Ali, Lee H. Lee, Nozrin Laskar, Nadia Shaikh, Hassan Tahir, Stephen D. Hsu, Robert Newby Jr., Jonathan Valsechi-Diaz, Tinchun Chu
KEYWORDS:
Bacterial Spores, Antimicrobials, Bacillus, Germination, Biocontrol
JOURNAL NAME:
Advances in Microbiology,
Vol.7 No.3,
March
9,
2017
ABSTRACT: Endospores have the ability to withstand extreme temperature, desiccation, ultraviolet radiation and chemicals which make them a threat to the food and healthcare industry. Green tea polyphenols (GTP), contain anti-microbial and anti-spore properties but not stable. In this study, two modified lipophilic green tea polyphenols, epigallocatechin-3-gallate-sterate (EGCG-S) and crude lipophilic green tea polyphenols (LTP), were used to compare their anti-spore effect with EGCG and crude GTP. Purified endospores from Bacillus cereus (B. cereus), B. megaterium and B. subtilis were treated with 1% or 5% of four tea polyphenols. Log reduction showed colony forming units (CFU) reduced significantly in all treated samples, ranging from 1.27 to 4.31 with no survivals (CFU = 0) in four samples (P