TITLE:
Mutagenicity of Wastewater Extracts from Pulp and Paper Industry
AUTHORS:
Samira Umme Aiman, Abdul Malik
KEYWORDS:
Mutagenicity, Pulp and Paper Industry, Wastewater Extracts
JOURNAL NAME:
Journal of Environmental Protection,
Vol.8 No.2,
February
28,
2017
ABSTRACT: Wastewater samples were
collected from pulp and paper mill located in Kashipur (India) and were
extracted using dichloromethane (DCM), chloroform and hexane solvents (all were
HPLC-grade). The extracts were assayed for their mutagenic potential using Ames Salmonella mutagenicity assay. TA98
strain was found to be the most responsive, in terms of induction factor (Mi)
and slope (m) of the initial linear dose-response curve as determined by linear
regression analysis up to the increasing doses indicating the presence of frame
shift mutagens in the test samples. Mutagenicity of different extracts is
arranged as follows: dichloromethane extracted water samples > hexane
extracted water samples > chloroform extracted water samples. Hexane extract
exhibited maximum mutagenic index of 13.0 and induction factor (Mi) 2.48 with
TA98. The order of responsiveness based on the mutagenic index and induction
factor for the test samples was in the following order: TA98 > TA97a > TA100 > TA102 > TA104.
Our findings suggest that TA97a, TA98, TA100, TA102, TA104 were sensitive
towards the wastewater extracts and showed considerable mutagenicity.