Ibiwoye, M.O., Powell, K.A., Grabiner, M.D., Patterson, T.E., Sakai, Y., Zborowski, M., Wolfman, A. and Midura, R.J. (2004) Bone Mass Is Preserved in a Critical-Sized Osteotomy by Low Energy Pulsed Electromagnetic Fields as Quantitated by in Vivo Micro-Computed Tomography. Journal of Orthopaedic Research, 22, 1086-1093.
https://doi.org/10.1016/j.orthres.2003.12.017

has been cited by the following article:

• TITLE: Effects of Pulsed Electromagnetic Fields of Different Frequencies on the Gene Expression of RANK and NFATc1 in Rat Osteoclasts Co-Cultured with Osteoblasts

  AUTHORS: Jian Chen, Hui Huang, Jianquan He, Zhang Xin

  KEYWORDS: PEMFs, Co-Cultured, RANK mRNA, NFATc1 mRNA

  JOURNAL NAME: Journal of Biomedical Science and Engineering, Vol.10 No.1, January 23, 2017

  ABSTRACT: The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) in rat osteoblast and osteoclast co-cultured model. Osteoblast-like cells were isolated from calvariae of Newborn Sprague Dawley rats (SD rats), while osteoclast-like cells were obtained from femora and tibiae of five weeks old SD rats. After 1 days of co-culture, the cells were exposed to premarin (E2) and different frequencies of PEMFs (8 Hz and 16 Hz, respectively) for 3 days. The expression of RANK and NFATc1 mRNA was analysed with realtime quantitative polymerase chain reaction. The gene expression of RANK and NFATc1 in the E2, PEMF with 8 Hz and 16 Hz group was significantly lower than that in the control group respectively. The gene expression of NFATc1 in the PEMF with 8 Hz group was significantly lower than that in the control group and PEMF with 16 Hz group. The study indicates that PEMF with 8 Hz could regulate the gene expression of RANK and NFATc1 in co-cultured model.