TITLE:
Effect of Activation of the Ca2+-Permeable Acid-Sensing Ion Channel 1a on Acid-Induced Vascular Endothelial Cell Injury of Henoch-Schönlein Purpura Children
AUTHORS:
Qidi Peng, Liping Yuan, Yan Bo, Xiaoyan Guo, Hu Bo
KEYWORDS:
Acid-Sensing Ion Channels (ASICs), Vascular Endothelial Cell, Henoch-Schönlein Purpura (HSP)
JOURNAL NAME:
Open Journal of Pediatrics,
Vol.6 No.4,
December
27,
2016
ABSTRACT: Acidosis
in local environment plays a critical role in cell injury. One key mediator of
acidosis-induced cell injury is the acid-sensing
ion channels (ASICs),
particularly ASIC1a. Herein, we investigated the role of ASIC1a in acid-induced
vascular endothelial cell injury of Henoch-Schonlein purpura (HSP) children. Acid-induced
ASIC1a, Calpain and Calcineurin expression in vascular endothelial cells
pretreated with IgA1 isolated from HSP were detected by real time quantitative polymerase chain
reaction and western blot methods, respectively. Cell cytotoxicity was measured
by interleukin-8 and nitric oxide production with ELISA. The results showed
acid-induced ASIC1a, Calpain and Calcineurin expression in cells increased, especially
at PH6.5. The cytotoxicity of vascular endothelial cells was increased by extracellular
acidosis. Moreover non-specific or specific blockers of ASIC1a, Amiloride and
PcTX-1 could remarkably decrease these parameters. These findings show that
increased [Ca2+]i, mediated via ASIC1a, might contribute to
acid-induced vascular endothelial cell injury of HSP.