TITLE:
Removal of Phenol Using Spore Forming Bacillus ABO11 Isolated from Waste Water Treatment Plant
AUTHORS:
Majdah Mohamed Ahmed Aburas
KEYWORDS:
Phenol, Degradation, Bacteria, Wastewater, Assay, Bacillus
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.12,
October
18,
2016
ABSTRACT: Twenty isolates of bacteria were isolated from
contaminated wastewater collected from wastewater treatment station, used to
clean the raw sewage at Jeddah on minimal medium containing phenol (0.5 g/l) as
carbon source while at 0.6 g/l phenol, five isolates belonging to Gram positive
and Gram negative bacteria were obtained after 10 days of growth at 37°C.
All the tested bacteria were tested for phenol degradation in liquid medium and
isolate ABO11 was the most active isolate in phenol degradation. It was selected
and tested for the ability to use phenol as carbon and energy source. It was
identified as Bacillus sterothermophilus ABO11 using morphological and
biochemical tests. Genetic studies confirmed the identification. Bacterial
growth and phenol degradation by the selected bacterium were determined using
different initial phenol concentrations (0 - 1.0 g/l). Very weak growth was
recorded at 0.8 g/l and no growth or degradation was observed at higher phenol
concentration. The isolate was adapted to grow in presence of 0.8 g/l phenol
and phenol degradation was checked up to 12 days of growth. Percentage of
degradation was 100% after 10 days. Maximum growth was observed at 40°C,
pH 8 and using NH4Cl as nitrogen source. In conclusion, the selected
isolate, Bacillus sterothermophilus ABO11 can be used in protecting the
environment from phenol pollution.