TITLE:
Metabolic Effects of Endogenous and Exogenous Heterotropic Hemoglobin Modulators on Anion Transport: The Case of Pig Erythrocytes
AUTHORS:
Ester Tellone, Annamaria Russo, Bruno Giardina, Antonio Galtieri, Silvana Ficarra
KEYWORDS:
Band 3 Protein, 2, 3-Diphosphoglycerate, Hemoglobin, Metabolism, Red Blood Cell
JOURNAL NAME:
Open Access Library Journal,
Vol.2 No.10,
October
21,
2015
ABSTRACT:
This comparative
study, focused on the anion kinetic of band 3 protein of pig erythrocytes, investigates in vitro the metabolic functionality
of the red blood cells of this mammal which show particular characteristics of
the cell membrane and significant stoichiometric variations of several
cytosolic components. Pig red blood cells compared to human ones, are
characterized by a higher anion flux strongly modulated by the conformational
transition state (T-R) of hemoglobin; these findings are probably connected to
the intracellular concentration of 2,3-disphosphoglycerate and to chloride
activity. The experimental use, alone or in combination, of some exogenous
heterotropic modulators of hemoglobin as orthovanadate, gemfibrozil and
resveratrol, shows characteristic modulations of the anion flux which are
related to the cytosolic metabolites physiologically present in pig erythrocytes,
to the metabolism and oxidative stress. Our findings highlight new features
about the pig red blood cells functionality and contribute to extending the
current understanding of their life cycle, providing opportunities for new
investigations in the field of comparative physiology.