TITLE:
Detection and Transfer of Extended Spectrum Beta Lactamase Enzymes from Untreated Hospital Waste Water
AUTHORS:
Olivia Sochi Egbule
KEYWORDS:
Waste Water, Plasmids, ESBL, Escherichia coli
JOURNAL NAME:
Advances in Microbiology,
Vol.6 No.7,
June
27,
2016
ABSTRACT: Untreated Hospital
wastewater piped into septic tanks contributes to the spread of antibiotic resistance
in developing countries. This study was conducted to determine the resistant
profile, and Extended Spectrum Beta-Lactamases (ESBLs) production in isolates
from hospital waste water, of 2 hospitals in Delta State, Nigeria. A total of
147 organisms were isolated from 32 waste water samples. One hundred and twenty
three isolates were Gram negative and 24 were Gram positive. Escherichia coli was the most prevalent
in the two locations. Antimicrobial susceptibility by standard disk diffusion
method was performed. All isolates were resistant to 4 or more antimicrobial
agents. Out of the 123 Gram negative Bacteria, 33 were pan drug resistant and
were selected for plasmid curing, DNA extraction and phenotypic detection of
ESBL. Transfer of resistant by broth mating technique was performed. Plasmid
curing and extraction result indicated that isolates carried resistance on the
plasmid and harboured similar multiple high molecular weight plasmids of 23.13 kb
and 9.4 kb. ESBL production was detected in 15.15%. Transfer of resistant genes
between ESBL producing and non-ESBL producing isolates was observed. Incidence
of ESBL in untreated hospital waste water has
public health implications. Therefore establishment of treatment plants in
our hospital is paramount in achieving sustainable health.