TITLE:
Role of Silver Nitrate on in Vitro Flowering and Shoot Regeneration of Solanum nigrum (L.)—An Important Multipurpose Medicinal Plant
AUTHORS:
G. Geetha, K. Harathi, C. V. Naidu
KEYWORDS:
In Vitro Flowering, Micropropagation, Silver Nitrate, Ethylene Inhibitor, Solanum nigrum
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.7 No.7,
May
11,
2016
ABSTRACT: Silver
nitrate induced MS media enhanced multiple shoot regeneration and in vitro flowering from axillary bud and
leaf explants of Solanum nigrum (L.)
an important antiulcer medicinal plant. Healthy axillary bud and leaf explants
were inoculated on MS medium supplemented with BAP/Kn (1.0 - 2.0 mg/l) in
combination with IAA/NAA (0.5 mg/l) and AgNO3 (0.1 - 1.0 mg/l). The
explants were responded effectively and good regeneration frequencies were
observed in all the combinations of silver nitrate tested when compared to
control. Maximum number of multiple shoots (34.3) was found in leaf explants
cultured on MS media supplemented with BAP (2.0 mg/l), NAA (0.5 mg/l) and AgNO3 (0.4 mg/l). These regenerated shoots were sub-cultured on to the flowering
media. Maximum number of in vitro flowers (12) was obtained from axillary bud explants in BAP (2.0 mg/l), Kn (1.0
mg/l), IAA (0.5 mg/l) and AgNO3 (6.0 mg/l) supplemented media when
compared to leaf (4) and control. All the in
vitro raised shoots were transferred to rooting medium supplemented with
NAA, IBA (1.0 - 2.0 mg/l) and AgNO3 (0.1 - 0.6 mg/l). The best
rooting response (24.6) was observed in 2.0 mg/l IBA + 0.4 mg/l AgNO3.
The well rooted plantlets were transferred to polybags containing soil +
vermiculate in 1:1 ratio for hardening and finally the hardened plantlets were
transferred to field conditions.