Bishop, G.J., Nomura, T., Yokota, T., Harrison, K., Noguchi, T., Fujioka, S., Takatsuto, S., Jones, J.D.G. and Kamiya, Y. (1999) The Tomato DWARF Enzyme Catalyses C-6 Oxidation in Brassinosteroid Biosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 96, 1761-1766. http://dx.doi.org/10.1073/pnas.96.4.1761

has been cited by the following article:

• TITLE: Physiological and Biochemical Effects of 24-Epibrassinolide on Heat-Stress Adaptation in Maize (Zea mays L.)

  AUTHORS: Pranjal Yadava, Jyoti Kaushal, Anuradha Gautam, Hemangini Parmar, Ishwar Singh

  KEYWORDS: Brassinosteroid, Maize, Heat-Stress

  JOURNAL NAME: Natural Science, Vol.8 No.4, April 8, 2016

  ABSTRACT: Brassinosteroids (BRs) are a family of about 70 structurally related polyhydroxy steroidal phytohormones that regulate a number of physiological processes in plants. Among these, brassinolide (BL), 28-homobrassinolide (28-homoBL) and 24-epibrassinolide (24-EpiBL) are more common. The present study aims at studying the usefulness of 24-epiBL in ameliorating the impacts of heat-stress in maize along with its role in regulating cellular antioxidant defense system. Maize hybrid PMH 3 was grown in pots in a green house maintained at 14 hours day (25℃)/10hours night (17℃). A solution of 24-epiBL (1 μM) was applied externally at V4 stage. Leaf tissues were sampled from both treated and control plants. Subsequently, both the groups of pots were placed in plant growth chamber maintained at high temperature (48℃; RH 50%). Plants were sampled for biochemical analysis after 3, 6, 9, 24 and 48 hours of high temperature exposure. Exogenous application of 24-EpiBL arrested protein degradation and enhanced cell membrane stability, as compared to the control. The biochemical activities of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD) and peroxidase (POX) were found to be dynamically and variably modulated post 24-epiBL treatment. Thus, the study supports the role of BRs as anti-stress agents.