Mpiana, P.T., Makelele, L.K., Oleko, R.W., Bokota, M.T., Tshibangu, D.S.T., Ngbolua, K.N., Mbala, M.B., Atibu, E.K. and Nsimba, S.M. (2010) Antisickling Activity of Medicinal Plants Used in the Management of Sickle Cell Disease in Tshopo District, D.R. Congo. Australian Journal of Medical Herbalism, 22, 132-137.

has been cited by the following article:

• TITLE: Chemical Fingerprint and Anti-Sickling Activity of Rosmarinic Acid and Methanolic Extracts from Three Species of Ocimum from DR Congo

  AUTHORS: Dorothée D. Tshilanda, Paulin K. Mutwale, Damase V. N. Onyamboko, Philippe B. Babady, Philippe V. Tsalu, Damien S. T. Tshibangu, Nadege K. Ngombe, M. Frederich, Koto-te-Nyiwa Ngbolua, Pius T. Mpiana

  KEYWORDS: Antisickling, Chemical Fingerprint, O. canum, O. basilicum, O. gratissimum, Rosmarinic Acid

  JOURNAL NAME: Journal of Biosciences and Medicines, Vol.4 No.1, January 14, 2016

  ABSTRACT: The aim of this study was to characterize the polyphenolic composition by determination of chemical fingerprints of Methanolic extracts of Ocimum canum Sims, Ocimum basilicum L. and Ocimum gratissimum L. from Democratic Republic of Congo and to compare their antisickling activity of that of rosmarinic acid, the major compound to those of methanolic extracts. Phytochemical analysis performed by TLC and HPLC analysis, showed that rosmarinic acid is the most abundant phenolic acid in these Ocimum species according to the following order O. basilicum L., O. gratissimum L. and O. canum Sims. Methanolic extracts of these three species and pure rosmarinic acid showed significant antisickling activities with minimal concentration of normalization values of 0.18 ± 0.03, 0.23 ± 0.04, 0.26 ± 0.04 and 0.31 ± 0.05 mg/mL for rosmarinic acid, O. basilicum L., O. gratissimum L. and O. canum Sims methanolic extracts respectively. The antisickling activity order is the same as that of the rosmarinic acid content, indicating that this polyphenolic acid would be among the main active molecules in these extracts.