TITLE:
A Study of the Cytotoxicity of a New Nonwoven Polymeric Fibrous Bandaging Material In-Vitro
AUTHORS:
A.M. Dygai, L.M. Ogorodova, S.G. Psakhie, Y.P. Belsky, N.V. Belska, M.G. Danilets, A.A. Ligatcheva, A.A. Сhurin
KEYWORDS:
cytotoxicity; bandaging materials, development of biocompatible biomaterials
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.2 No.3,
May
20,
2011
ABSTRACT: Traditionally used cotton-based bandaging materials have several disadvantages which can be overcome by using another fabric structure – nonwoven fabric. Moreover, these materials are more spongeous which increases their sorption capacity. The new bandaging material developed by the Institute of Physics of Strength and Material Science of the Siberian Branch of the Russian Academy of Sciences has even better sorption capacity with improved sorption properties. Its sorption capacity has been increased by means of an additional introduction of porous aluminum hydrate particles into fabric. It is important that a bandaging material has a good biocompatibility and does not have any cytotoxic effect on cells and tissues. Here it is present results of the study of the material's direct contact and indirect cytotoxicity assays in comparison with cotton gauze. It have found that in the direct contact of nonwoven polymeric fibrous bandaging material (NPFBM) with cells for 24 hours of cultivation no changes in cell morphology take place, nor does the amount of dead cells increase. These conclusions have been made by means of both a visual examination and an МТТ assay. The NPFBM extract did not have any cytotoxic effect on the tested cells either. The obtained results allow us to make a conclusion that the NPFBM complies with the international standard ISO 10993-5, which is applied to medical goods, and can from now on be applied in the treatment of infected wounds in clinical practice.