TITLE:
Rubber Seed Kernel as Potent Solid Substrate for the Production of Lipase by Pseudomonas aeruginosa Strain BUP2
AUTHORS:
K. N. Unni, Panichikkal Abdul Faisal, Prakasan Priji, Sreedharan Sajith, Sreedharan Sreedevi, E. S. Hareesh, Trikariyoor Asokan Nidheesh Roy, Sailas Benjamin
KEYWORDS:
Pseudomonas aeruginosa BUP2, Lipase, Solid-State Fermentation, BUP Medium, Rubber Seed Flour
JOURNAL NAME:
Advances in Enzyme Research,
Vol.3 No.2,
June
10,
2015
ABSTRACT: This study explored the utility of flours of rubber seed, coconut and groundnut kernels, and de-oiled cakes of coconut and groundnut as solid substrate for the production of lipase by Pseudomonas aeruginosa strain BUP2 (MTCC No. 5924), a novel bacterium reported from the rumen of Malabari goat. Various proportions (10%, 20%, 30%, 40% or 50%) of flours or cakes were prepared (w/v) with BUP medium (pH 4, 5, 6, 7 or 8), and incubated at different temperature (25°C, 28°C, 30°C or 32°C) for 24 to 96 h. The samples were assayed for lipase activity at 24 h intervals. The rubber seed flour (20%)-BUP medium supported the maximum lipase production (871 U/gds) at 48h incubation (pH 6, 28°C), followed by ground nut flour (398 U/gds), while ground nut cake supported the least lipase production (244 U/gds). From this, it is evident that the cheaply available rubber seed is an efficient substrate for the production of lipase, irrespective of its known demerit that it contains the limarin, a toxin; in fact, we could not detect limarin in the fermented matter. Thus, the utility of rubber seed for the production of a costly enzyme is reported from a novel rumen bacterium, which would be advantageous for rubber farmers.