TITLE:
In Vitro Inhibitory Activity of the Leaf Methanol Extract of Green Tea (Camellia sinensis) against Lactococcus garvieae and Aeromonas hydrophila Isolated of Rainbow Trout (Oncorhynchus mykiss)
AUTHORS:
P. Akbary
KEYWORDS:
Antibacterial Activity, Camellia sinensis, Disc Diffusion, Plant Extract
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.12,
September
23,
2014
ABSTRACT: Camellia sinensis Linn is a well-known
medical herb that grows in various parts of the world. In the current study,
the antimicrobial activity of methanol extract from C. sinensis leaves
against Lactococcus garvieae and Aeromanas hydrophila isolated
from rainbow trout (Oncorhynchus mykiss) was investigated. The growth
inhibitory effects of green tea extract was determined by disc diffusion method
(3 times on different days), minimum inhibitory concentration (MIC) using
serial dilution and minimum bactericidal concentration (MBC). For the L.
garvieae and A. hydrophila, the zone diameter inhibition (ZDI) of
extract ranged 2.30 - 16.5 mm. The highest ZDI (16.50 ± 1.12 mm) for L.
garvieae was observed at 100 mg•ml-1 and for A.
hydrophila (16.20 ± 0.95 mm) at 250 mg•ml-1 concentration of
green tea extract (P •ml-1 concentrations of
extract, ZDI values of A. hydrophila and L. garvieae showed
significant difference (P •ml-1 against both L.
garvieae and A. hydrophila. From the results of the present study,
it can be concluded that methanol extract of C. sinenses leaves could be
effective for the inhibition of A. hydrophila and L. garvieae in
rainbow trout.