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http://dx.doi.org/10.3390/md8030399

has been cited by the following article:

• TITLE: Cultivable Sponge-Associated Actinobacteria from Coastal Area of Eastern Mediterranean Sea

  AUTHORS: Özlem Öner, Güner Ekiz, E. Esin Hameş, Volkan Demir, Özkan Gübe, F. Can Özkaya, M. Baki Yokeş, Ataç Uzel, Erdal Bedir

  KEYWORDS: Sponge-Associated Actinobacteria, Streptomyces, Nocardiopsis, Antimicrobial Activity

  JOURNAL NAME: Advances in Microbiology, Vol.4 No.6, May 13, 2014

  ABSTRACT: In this study, 16 marine sponge samples collected from Eastern Mediterranean Sea were used for the isolation of sponge-associated Actinobacteria. Different media and pre-treatment methods were applied for the isolation experiments. In total, 7578 actinomycetes colonies were observed on the primary isolation plates, and 20 representative isolates were selected for further studies based on their morphologies and chemical profiles. These isolates were investigated for their antimicrobial activities, seawater requirements and 16S rRNA gene sequences. The phylogenetic analysis revealed that 80% of the isolated Actinobacteria was a member of the Streptomyces genus. All the isolates showed no absolute requirement for seawater. Fifteen of the selected isolates exhibited antimicrobial activity against methicillin resistant Staphylococcus aureus and vancomycin resistant Enterococcus faecium, whereas 3 isolates were active against Candida albicans.