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has been cited by the following article:

• TITLE: GenoType MTBDRplus as a Complementary Tool for the Typing of Mycobacterium tuberculosis

  AUTHORS: Timothée Ouassa, Guillaume Yao Loukou, Hortense Faye-Kette

  KEYWORDS: Mycobacterium tuberculosis; MIRU-VNTR; Spoligotyping; GenoType MTBDRplus; Côte d’Ivoire

  JOURNAL NAME: Advances in Infectious Diseases, Vol.4 No.1, March 4, 2014

  ABSTRACT: The aim of this study was to investigate the usefulness of combining profiles obtained by using a line probe assay (LPA) originally intended to characterize the resistance of two major anti-tuberculosis drugs to the association of spoligotyping and MIRU-VNTR, in order to improve its discriminatory power. For this purpose, 74 strains of Mycobacterium tuberculosis belonging to the same cluster after spoligotyping were further typed by using the 24 loci MIRU/VNTR. These strains were then tested by the GenoType MTBDRplus, and profiles obtained were analyzed within previously obtained clusters. The combination of spoligotying and MIRU-VNTR led to the consolidation of 56 of them (75.7%) in 9 clusters. Most of the strains (54, 96.4%) were multidrug resistant (MDR). From the 9 initial clusters, the addition of GenoType MTBDRplus helped to define 26 profiles including 11 unique profiles, and 3 original clusters remained undifferentiated. Results obtained express the relevance of combining this method which improved quite significantly the discriminatory power in typing Mycobacterium tuberculosis.