TITLE:
Polysaccharides as initiators of nucleic acid polymerization
AUTHORS:
Aleksander N. Zimnitskii, Sergey A. Bashkatov, Aleksey V. Chemeris, Renat S. Yamidanov, Vadym N. Urazbaev
KEYWORDS:
Polysaccharides; Nucleic Acids; Polymerization; Complementarity; PCR
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.4 No.1,
January
3,
2014
ABSTRACT:
Quantum-chemical calculations and in vitro experiments have demonstrated
that polysaccharides are able to adsorb mononucleotides due to the formation of
hydrogen bonds. The subsequent dehydration of polysaccharide-nucleotide
complexes in the physiologically acceptable temperature range results in the
creation of “correct” DNA polymers that are “recognized” by the specific
DNA-polymerases. DNA fragments abiogenically formed on polysaccharides vary in
size and are characterized by a relatively “simple”, most probably, tandem
structure. This research developed our previous concept of template-based polysaccharide
synthesis with the participation of DNA tandem repeats (glycotranscription
concept) making it possible to assume the existence of reverse glycotranscription
in biological systems. Future research in the direction that we outlined
experimentally may lead to a revolutionary approach in modern molecular biology—glycogenomics.