TITLE:
Characterization of Pectin Nanocoatings at Polystyrene and Titanium Surfaces
AUTHORS:
Katarzyna Gurzawska, Kai Dirscherl, Yu Yihua, Inge Byg, Bodil Jørgensen, Rikke Svava, Martin W. Nielsen, Niklas R. Jørgensen, Klaus Gotfredsen
KEYWORDS:
Surface Properties; Titanium; Polystyrene; Rhamnogalacturonan-I; Osseointegration
JOURNAL NAME:
Journal of Surface Engineered Materials and Advanced Technology,
Vol.3 No.4A,
October
22,
2013
ABSTRACT:
The titanium implant surface plays a
crucial role for implant incorporation into bone. A new strategy to improve implant integration in a bone is to develop surface
nanocoatings with plant-derived polysaccharides able to increase adhesion of bone cells to the implant
surface. The aim of the present study was to physically characterize and compare polystyrene and titanium surfaces
nanocoated with different Rhamnogalacturonan-Is (RG-I) and to visualize RG-I
nanocoatings. RG-Is
from potato and apple were coated on aminated surfaces of polystyrene, titianium discs and titanium
implants. To characterize, compare and visualize the surface nanocoatings
measurements of contact angle measurements and surface roughness with atomic force microscopy, scanning
electron microscopy, and confocal microscopy was performed. We found that, both
unmodified and enzymatic modified RG-Is influenced surface wettability, without
any major effect on surface
roughness (Sa, Sdr). Furthermore, we demonstrated that it is possible to
visualize the pectin RG-Is molecules and even the nanocoatings on titanium
surfaces, which have not been presented before. The comparison between polystyrene and titanium surface showed that the used
material affected the physical properties of non-coated and coated surfaces. RG-Is should be considered as a candidate for
new materials as organic nanocoatings for biomaterials in order to improve bone
healing.