TITLE:
Leptin Receptor in Ram Epididymal Spermatozoa
AUTHORS:
Mohammad Roostaei-Ali Mehr, Reza Rajabi-Toustani, Rasool Motamedi-Mojdehi
KEYWORDS:
Epididymal Spermatozoa; mRNA; Ob-R; Ob-Rb
JOURNAL NAME:
Engineering,
Vol.4 No.10B,
October
21,
2013
ABSTRACT:
This experiment was
designed to investigate leptin receptors (Ob-R and Ob-Rb) mRNA in ram
epididymal spermatozoa by RT-PCR. Ten testes were obtained from abattoir and
epididymal spermatozoa recovery was performed. To purify spermatozoa, motile
sperm were isolated by the swim-up procedure. Total RNA was isolated from
epididymal spermatozoa and placental cotyledon, as a positive control, and then
they were purified. Specific bands (98 and 308 bp) for Ob-R and Ob-Rb were
detected after RT-PCR in both epididymal spermatozoa and placental cotyledon.
We may conclude that Ob-R and Ob-Rb mRNA are present in ram epididymal
spermatozoa and leptin perhaps exerts physiological effects, as already demonstrated
in human.