TITLE:
To Optimize the Therapeutic Dose and Time Window of Picroside II in Cerebral Ischemic Injury in Rats by Orthogonal Test
AUTHORS:
Hui Huang, Li Sun, Ling Wang, Lei Fang, Li Zhao, Yan Li
KEYWORDS:
Picroside II; Cerebral Ischemia; Therapeutic Dose; Time Window; Rats
JOURNAL NAME:
Neuroscience and Medicine,
Vol.4 No.3,
September
11,
2013
ABSTRACT:
The paper aims to optimize
the therapeutic dose and time window of picroside II by orthogonal test in cerebral
ischemic injury in rats. The forebrain ischemia models were established by
bilateral common carotid artery occlusion (BCCAO) methods. The successful
models were randomly divided into sixteen groups according to orthogonal
experimental design
and treated by injecting picroside II intraperitonenally at different
ischemic time with different dose. The
concentrations of
neuron-specific enolase (NSE), neuroglial marker protein S100B and myelin basic
protein (MBP) in serum were determined by enzyme linked immunosorbent assay to
evaluate the therapeutic effect of picroside II in cerebral ischemic injury. The
results indicated that best therapeutic time window and dose of picroside II in cerebral ischemic injury were
ischemia 1.5 h
with 20 mg/kg body weight according to the
concentrations of NSE, S100B and MBP in serum. It is
concluded that according to the principle of lowest therapeutic dose with
longest time window, the optimized therapeutic dose and time window are injecting picroside II intraperitonenally with 20 mg/kg body weight at ischemia 1.5 h in cerebral ischemic injury in rats.