K. Hossain, A. A. Akhand, M. Kato, J. Du, K. Takeda, J. H. Wu, K. Takeuchi, W. Liu, H. Suzuki and I. Nakashima, “Arsenite Induces Apoptosis of Murine T Lymphocytes through Membrane Raft-Linked Signaling for Activation of c-Jun Amino-Terminal Kinase,” Journal of Immunology, Vol. 165, No. 8, 2000, pp. 4290-4297.

has been cited by the following article:

• TITLE: Cytotoxic Activity of Thelesperma megapotamicum Organic Fractions against MCF-7 Human Breast Cancer Cell Line

  AUTHORS: Ana Cristina Figueroa, Elio Andres Soria, Juan Jose Cantero, Mónica Silvina Sanchez, Marta Ester Goleniowski

  KEYWORDS: Thelesperma megapotamicum; Cancer Breast; MCF-7 Cells; Apoptosis; Membrane Syalization; Gama-Glutamyltranspeptidase Activity

  JOURNAL NAME: Journal of Cancer Therapy, Vol.3 No.1, February 17, 2012

  ABSTRACT: Thelesperma megapotamicum (Asteraceae) is commonly used in Argentine to treat various diseases (renal, digestive affections, and as anaesthesia). The present study showed the mechanisms involved “in vitro” cytotoxicity of T. megapotamicum Fractions. Five Fractions (F1 - F5) were separated by column chromatography (Silica gel) using hexane:diethyl ether as eluents. Viability was evaluated in Human breast carcinoma cell line (MCF-7) by staining with crystal violet. With respect to F1 Fraction treatment, the cell survival was 49.14% ± 8.87%, while the F2 and F3 ones exhibited a strong reduction of cell viability to only 26.35% ± 1.63% and 23.3%1 ± 0.53% of the control cell at 50 μg/ml, respectively. Apoptotic effect of these Fractions was detected using FITC-labeled Annexin V and propidium iodide binding assays and was confirmed by a higher proportion of apoptotic cells due to F2 and F3 treatments. T. megapotamicum active Fractions could facilitate the tumoral cells death by decreasing the activity of the enzyme Gamma-glutamyltranspeptidase and causing alteration in cell membrane sialoglycoconjugates and others involved anticancer mechanisms including apoptosis.