TITLE:
Enrichment and Isolation of CO2-Fixing Bacteria with Electrochemical Reducing Power as a Sole Energy Source
AUTHORS:
Bo Young Jeon, Il Lae Jung, Doo Hyun Park
KEYWORDS:
Electrochemical Reducing Power; CO2; Autotroph; Alcaligenes Sp; Pyrosequencing
JOURNAL NAME:
Journal of Environmental Protection,
Vol.3 No.1,
January
5,
2012
ABSTRACT: Enrichment of bacteria capable of growing with electrochemical reducing power and CO2 was accomplished using a plate-type electrochemical bioreactor (PEB). A bacterial source obtained from wastewater treatment reactant and forest soil was cultivated on carbonate-based mineral agar medium prepared in the PEB (PEB-carbonate agar). According to the pyrosequencing analyses, the abundance of Betaproteobacteria and Gammaproteobacteria at the phylum level, and Achromobacter, Alcaligenes, and Pseudomonas at the genus level were selectively increased after the electrochemical enrichment culture. Finally, one genus of bacterium that was autotrophically grown on the PEB-carbonate agar was identified as Alcaligenes. This bacterium may be useful to fix atmospheric CO2 with electrochemical energy obtained from the solar cell.