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https://doi.org/10.18632/oncotarget.27037

has been cited by the following article:

• TITLE: Low Level of Cystic Fibrosis Transmembrane Conductance Regulator Is Associated with Human Sperm Autophagy and Vitality

  AUTHORS: Jie Hu, Han Liu, Chaoyan Ou, Liangzhao Liu, Linfeng Mo, Xuming Liang, Yonghua He

  KEYWORDS: Autophagy, CFTR, Sperm, Vitality

  JOURNAL NAME: Advances in Reproductive Sciences, Vol.12 No.1, February 22, 2024

  ABSTRACT: Low sperm motility is one of the main causes of male infertility. Cystic fibrosis transmembrane conductance regulator (CFTR, an anion channel protein) is related to the progressive motility of sperm. CFTR disruptor CFTRinh-172 or forskolin (FSK) in this study were used to treat human sperm separately, and the rates of sperm autophagy and progressive motility, mitochondrial membrane potential (MMP) and ATP concentration, and the expression levels of related factors were detected to explore their relationship. It was showed that sperms treated with CFTRinh-172 or FSK reduced the levels of cAMP, CFTR and PKA, but increased sperm autophagy rate, expression levels of AMPK and LC3B. However, reactive oxygen species content had no significant difference. It was indicated that low level of CFTR performed with cAMP and its downstream effectors such as PKA and AMPK to regulate mitochondrial structure and function, leading to increased autophagy rate and reduced vitality of sperm.