TITLE:
Therapeutic Approach for Hair Growth and Regeneration Using Bioactive Formulation Containing Mesenchymal Stromal Cell-Derived Conditioned Medium
AUTHORS:
Pawan Kumar Gupta, Samatha Bhat, Suresh Kannan, Raviraja N. Seetharam, Udaykumar Kolkundkar
KEYWORDS:
Trichosera®, Bone Marrow Derived Mesenchymal Stromal Cells, Conditioned Media, Hair Fall, Hair Regrowth, Human Volunteer Study
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.13 No.3,
September
13,
2023
ABSTRACT: Background and Aims: Androgenetic alopecia (AGA) is a common form of hair
loss in both men and women. Despite its high prevalence and associated patient
morbidity, the approved therapeutic options are limited to finasteride and
minoxidil. The present study is aimed at assessing the efficacy of hair serum
formulation, Trichosera?containing Bone marrow-derived mesenchymal stromal
cells conditioned media as an active ingredient, for hair fall control and hair
regrowth in healthy Indian human volunteers. Methods: The product was made using a 20% concentration of
10X Conditioned Media along with excipients. The final product was tested for
physicochemical parameters, biomarkers, total protein content and microbial
limits as per our in-house specifications. Results: The primary irritation patch test showed that the
product is non-irritant and dermatologically safe. A clinical study on 40
subjects was conducted to evaluate the effectiveness of the bioactive formulation
in hair fall control and hair regrowth in healthy volunteers. Phototrichogram
measurement showed hair density and hair growth rate increased significantly by
11.54% and 18.66% at week 24. Hair tensile strength also increased significantly
by 41.10% at 12 weeks follow-up. Hair pull test, to see a reduction
in pulled hair and comb’s test to show a decrease
in hair fall significantly improved from week 4 onwards. There were no significant adverse events in response to the product application. Conclusion: It is concluded that the hair serum product is
completely safe on direct application to the scalp and showed significant improvement in the hair growth rate, hair density,
scalp condition and reduction in hair fall.