TITLE:
Effects of Methanolic and Aqueous Extracts of Griffonia simplicifolia (Fabaceae) on the Inhibition of Falciformation of Human Hb SS Erythrocytes
AUTHORS:
Joel Akakpo-Akue, Gnamien Marcel Ahon, Tatiana Kangah Mireille Kplé, Loukou Marie Louise Djaha, Fofie Yvette, Sanogo Ibrahime, Bahi Calixte, Djaman Allico Joseph
KEYWORDS:
Griffonia simplicifolia, Antisickling, Aqueous Extract, Methanolic Extract, SS Erythrocyte
JOURNAL NAME:
Open Journal of Applied Sciences,
Vol.13 No.1,
January
13,
2023
ABSTRACT: Sickle cell disease (SCD) is a genetic blood disorder that affects the
shape and transportation of red blood cells (RBCs) in blood vessels, leading to
various clinical complications. Sickle cell disease is a widespread genetic
disease in Black Africa. The objective of this study was to evaluate the
sickling inhibition activity of Griffonia simplicifolia. Quantitative and qualitative tests were used to determine the major
groups of secondary metabolites present in the Griffonia simplicifolia leaves
extracts and the modified Emmel test was used to perform the study of sickling
inhibition activity. The OECD 423 toxicity study showed that at a single dose
of 2000 mg/kg bw, Griffonia simplicifolia leaves extracts are not toxic.
All tested substances inhibit erythrocyte falciformation in a dose-dependent
manner. The percentages of inhibition were 50.35%, 73% and 94.23% for aqueous
extract, hydromethanolic extract and phenylalanine respectively at the
concentration of 15 mg/mL. The methanolic extract (70%) had higher activity
compared to the aqueous extract. These
results suggest that the Griffonia
simplicifolia extracts have some potential to be used as alternative
antisickling therapy in SCD management.