TITLE:
Identification and Analysis of miRNAs and siRNAs in Botrytis cinerea
AUTHORS:
Yaoyao Liu, Miao Sun, Qinru Yu, Ran Li, Xu Hu, Pengcheng Zhang, Tongfei Lai, Ting Zhou
KEYWORDS:
Botrytis cinerea, MicroRNA, Small Interfering RNA, Biological Function
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.13 No.5,
May
27,
2022
ABSTRACT: Small RNAs in Botrytis
cinerea were analyzed via high-throughput sequencing on BGISEQ-500
platform. A total of 8 novel miRNAs and 110 novel siRNAs were predicted.
Sequence information, construction, length distribution, base bias and
expression levels of miRNAs and siRNAs were determined as well. Through GO and
KEGG enrichment analysis, the miRNA target genes are mostly located in membrane
and organelle, possessed binding and catalytic activities, and involved in
signal transduction and carbohydrate metabolism. The results will provide a
theoretical foundation for understanding the developmental and pathogenic
mechanisms of B. cinerea at the transcriptional level.