TITLE:
Molecular Genotyping of Human Papillomaviruses (HPV) in HIV+ and HIV− Women in Senegal
AUTHORS:
Babacar Faye, Serigne A. A. M. Gueye, Jean A. D. Tine, Hameth Sarr, Alioune Dièye
KEYWORDS:
HPV, Prevalence, Genotypes, HIV, PCR
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.12 No.2,
April
28,
2022
ABSTRACT: Background: Different studies have demonstrated high prevalence of HPV infection and dysplastic lesions of the cervix in immunocompromised patient such as women living with HIV. Is this high prevalence due to a greater susceptibility to HPV infection, which is known to be frequent in its latent form in women? Objective: This study aims to identify HPV genotypes in HIV+ and HIV− women to understand HPV molecular epidemiology in Senegal. Material and Method: Endocervical samples from 331 HIV+ and HIV− women, sexually active, were collected. The molecular identification of the 28 genotypes studied (19 HPV-HR and 9 HPV-LR) was carried out after DNA extraction, by multiplex PCR with the Anyplex™ II HPV28 detection kit from Seegene on CFX96™ Bio-Rad machine. The comparisons were made by calculating the p-value and odds ratio with R Studio software (version 4.1.0). The results were considered significant if p Results: The general prevalence of HPV was significantly higher in HIV+ women with 78.95% vs 64.65% for HPV; 72.18% vs 57.07% for HPV-HR; 57.14% vs 34.34% for HPV-BR (p − women. Conclusion: Our results showed that the prevalence of HPV, HPV-HR and HPV-BR was significantly higher in HIV+ women. Non-vaccine genotypes were among the most found genotypes. Groups of HIV+ women aged between 35 and 50, married and using contraception were significantly more infected with HPV than the same groups of HIV-women.