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has been cited by the following article:

• TITLE: Triploid Wild Rice (BKK) Strain Found in Bangkok Originated from Hybridizations among Three Parental Oryza Species

  AUTHORS: So Makabe, Htet Aung Htut, Hiroko Takahashi, Sayaka Shida, Masahiro Akimoto, Hathairat Urairong, Ryuji Ishikawa, Tadashi Sato, Yo-Ichiro Sato, Ikuo Nakamura

  KEYWORDS: Oryza rufipogon, Triploid, Flow Cytometry, PolA1, CentO-C1 Repeat Sequence

  JOURNAL NAME: American Journal of Plant Sciences, Vol.13 No.1, January 14, 2022

  ABSTRACT: A wild rice (BKK) strain showing sterile spikelet and big leaves inhabited at the basin of the Chao Phraya river of Bangkok city, Thailand. The BKK strain was found as a natural triploid and thus its origin has been interested long time. Three different-sized fragments were amplified in RNA polymerase I largest subunit (PolA1) gene, which is a single-copy nuclear gene per haploid genome. Short type (0.14 kb) intron 20 sequence of BKK strain was identical to that of O. rufipogon and O. sativa. Phylogenetic analysis showed that long type (1.5 kb and 1.8 kb) intron 20 sequences of BKK strain were closely related to that of O. longistaminata and O. officinalis, respectively. We analyzed protein tag (Ptag) sequence encoded by exons 19 to 21 of PolA1 gene. Determined three Ptag sequences of BKK strain were identical to that of O. rufipogon, O. longistaminata, and O. officinalis, respectively. Relative DNA content of nuclei in O. officinalis and BKK strain was 1.5 and 1.75 times than that in O. sativa, respectively. And BKK strain contained CentO-C1 repeats, which were unique to O. officinalis. These results indicated that BKK strain comprised three genomes of O. rufipogon, O. longistaminata, and O. officinalis.