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has been cited by the following article:

• TITLE: Investigation on Viral Pathogens in Vitis vinifera from Four Production Bases in Hangzhou Vicinity of China by sRNAseq and Molecular Validation

  AUTHORS: Hongmei Li, Lingzhu Wei, Jiang Wu, Nongnong Shi

  KEYWORDS: Vitis vinifera, Viral Disease, Small RNA Deep Sequencing, RT-PCR

  JOURNAL NAME: American Journal of Plant Sciences, Vol.12 No.12, December 22, 2021

  ABSTRACT: This is the first systematic investigation of viral pathogens in Vitis vinifera from Hangzhou vicinity of China. About 7 viruses and 5 viroids were annotated from four production bases “Dushicun”, “Wangjiayuan”, “Xiajiangcun”, and “Yangducun” covering 15 cultivars through sRNAseq technique. At least 3 viruses—grapevine leaf roll-associated virus 3 (GLRaV-3), grapevine fleck virus (GFkV) and grapevine geminivirus A (GGVA), and 4 viroids—hop stunt viroid (HSVd), citrus viroid II (CVd-II), grapevine yellow speckle viroid 1 (GYSVd-1) and grapevine yellow speckle viroid 2 (GYSVd-2) infected all four bases. “Yangducun” base showed 11, the most infected pathogens. GYSVd-1 showed the highest accumulation in host of Wangjiayuan base. The main infected pathogens were verified by reverse-transcription polymerase chain reaction (RT-PCR) technique, the detected rate reached to 85% - 100%. The results provide an important basis for effective and precise detection of viral diseases in the area and for the virus-free cultivation in future.