TITLE:
Fermentative Biohydrogen Production with Enteric Bacteria Isolated from the Intestine of Wild Common Carp Dwelling in Tarim River Basin
AUTHORS:
Yanbin Li, Qin Zhang, Hui Jiang
KEYWORDS:
Fermentative Biohydrogen Production, Enteric Bacteria, Intestine, Cotton Stalk Hydrolysate, Wild Common Carp
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.11 No.9,
September
17,
2020
ABSTRACT: The biological hydrogen generating from fermentation
of low-cost lignocellulosic feedstocks by hydrogen-producing bacteria has
attracted many attentions in recent years. In the present investigation, ten
hydrogen-producing bacteria were newly isolated from the intestine of wild
common carp (Cyprinus
carpio L.), and identified belonging to the genera of Enterobacter and Klebsiella based on analysis of the 16S rDNA gene sequence and
examination of the physiological and biochemical characteristics. All the
isolates inherently owned the ability to metabolize xylose especially the
cotton stalk hydrolysate for hydrogen production with hydrogen yield (HY)
higher than 100 mL·L-1. In particular, two isolates, WL1306 and WL1305 obtained
higher HY, hydrogen production rate (HPR), and hydrogen production potential
(HPP) using cotton stalk hydrolysate as sugar substrate than the mixed sugar of
glucose & xylose, which obtained the HY of 249.5 ± 29.0, 397.0 ± 36.7 mL·L-1, HPR of 10.4 ± 1.2, 16.5 ± 1.5 mL·L-1·h-1, HPP of 19.5 ± 2.3, 31.0 ± 2.8 mL·L-1·g-1sugar, separately. The generation of soluble metabolites,
such as the lactate, formate, acetate, succinate and ethanol reflected the
mixed acid fermentation properties of the hydrogen production pathway.