TITLE:
Intrinsic Bio-Enhancer Entities of Fagonia cretica for Synthesis of Silver Nanoparticles Involves Anti-Urease, Anti-Oxidant and Anti-Tyosinase Activity
AUTHORS:
Aqsa Yousaf, Ayesha Zafar, Muhammad Ali, Snober Mona Bukhary, Yasmeen Manzoor, Tuba Tariq, Asma Saeed, Muhammad Akram, Faryal Bukhari, Muhammad Abdullah, Syeda Sadaf Zehra, Shahbaz Gul Hassan, Murtaza Hasan
KEYWORDS:
Bioactivity, Fagonia cretica, Nanoparticles, Enzymes Assay, Bio Reducing Agent
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.10 No.12,
December
25,
2019
ABSTRACT:
Objective: Evaluating the bio-reducing activity and various
potential, annotates the biological entitties of Fagonia cretica. Method: By fractionating the crude extract
with different ethanol-water fraction mixture. This substantiates the reduction
potential for the biological synthesis of AgNPs, its antioxidant, anti-urease
and anti-Tyrosinase activity. Results: UV-visible spectra confirm the
bio-reducing potential for Ag NPs with the optimized 50% ethanol-water fraction
mixture. Transmission electron microscopy (TEM) confirmed the round shaped
AgNPs with an average size of 16 nm in optimized 50% fraction mixture. Further
evaluating Ethanol-water fraction with 90% and 70% of plant extract showed more
potentials for Anti-Tyrosinase and Anti-urease activity. Moreover, diluted 30%
fraction exhibits two folds higher Anti-oxidant and Anti urease activity. Conclusion: Overall, this work showed that 50% Ethanol-water fraction has potentially
active molecules (among all fractions) primarily involved in bio-reducing
potential of Ag NPs and enzymatic assay while 90% mixture proved to be least
active. This study suggested electing the optimum mixture fraction of 50%
ethanol-water in order to screen the bioactive compounds and to inculcate their
activity status for clinical trials.