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has been cited by the following article:

• TITLE: Regeneration of in Vitro Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

  AUTHORS: A. R. Zuraida, Kumeran G. Sentoor, N. Ahmad, Farhanah M. S. Syahirah, Nazreena O. Ayu

  KEYWORDS: Hormone Manipulation, Plant Growth Regulator, MATAG Coconut, Zygotic Embryo, Callus Induction

  JOURNAL NAME: American Journal of Plant Sciences, Vol.8 No.3, February 4, 2017

  ABSTRACT: Zygotic embryos tissues have found to be more responsive explants for clonal propagation of coconut. In the present study, the feasibility of using zygotic embryos explants for clonal propagation of a local coconut variety MATAG F2 was assessed. Callus was induced by incorporating of cytokinin and auxin into the medium. The sliced embryos explants were immersed in 1 M maltose for 60 mins, then with 0.05 M maltose for 1 min and followed by 0.01 M maltose for 5 mins was the best for prevention of phenolic compounds excretion. The callus formation depended on the concentration of 2,4-D in the media and the best effect was observed with the high level (2,4-D and BAP) tested. Attempts at inducing multiple shoot from the zygotic embryos callus were unsuccessful. No multiple shoots was present; most of the callus became root structure.