Article citationsMore>>
Drosten, C., Panning, M., Drexler, J.F., Hänsel, F., Pedrosoc, C., Yeats, J., de Souza Luna, L.K., Samuel, M., Liedigk, B., Lippert, U., Stürmer, M., Doerr, H.W., Brites, C. and Preiser, W. (2006) Ultrasensitive Monitoring of HIV-1 Viral Load by a Low-Cost Real-Time Reverse Transcription-PCR Assay with Internal Control for 5’ Long Terminal Repeat Domain. Clinical Chemistry, 52, 1258-1266.
http://dx.doi.org/10.1373/clinchem.2006.066498
has been cited by the following article:
-
TITLE:
Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B
AUTHORS:
Erick Ntambwe Kamangu, Adawaye Chatte, Raphael Boreux, Fabrice Susin, Richard Lunganza Kalala, Georges Lelo Mvumbi, Patrick De Mol, Marie-Pierre Hayette, Dolores Vaira
KEYWORDS:
COBAS AmpliPrep/TaqMan, In-House PCR Assays, HIV-1 Non-B
JOURNAL NAME:
Open Access Library Journal,
Vol.2 No.3,
March
20,
2015
ABSTRACT:
Context: The in-house techniques or experimental methods are increasingly
recommended for their low-cost reagents for the determination of the Viral Load
(VL) in resource-limited settings. The objective of this study was to compare
the determination of VL from HIV-1 non-B samples by an in-house technique with
the COBAS AmpliPrep/TaqMan version 2.0. Method: In this cross-sectional
study, 39 plasma samples from patients infected with HIV type 1 non-B from N’Djamena
and Kinshasa were used to determine the VL using the two techniques. Results: The
mean values of VL are respectively 4.68 ±
1.26 and 4.58 ± 1.33 log10 RNA copies/ml for the COBAS AmpliPrep/TaqMan
assays and the in-house assays. A good correlation (Spearman Correlation) was
obtained, with a coefficient (R2) of 0.9452. Conclusion: This study
demonstrates that there is no significant difference between the results of VL
determined by the COBAS AmpliPrep/TaqMan assays and the in-house assays used.
Related Articles:
-
Lihanimpo Djalogue, Mossi Komi Edem, El Hadj Yacoubou, Tchamdja Toyi, Djagadou Kodjo Agbeko, Balaka Abago, Djibril Mohaman Awalou
-
Sarah Tasneem, Lester Lipsky, Reda Ammar, Howard Sholl
-
Ali H. Yasir, Alaa S. Khalaf, Moayad N. Khalaf
-
Tiama Guy Nicaise Ballet, Kôkôh Rose Effebi, Olga Yolande Aké Ako, Yapo Habib Kpidi, Ossey Bernard Yapo
-
Hervé A. N’cho, Innocent K. Kouame, Kouadio Koff, Lazare K. Kouassı, Séraphin K. Konan, Ruth Baï, Andrei Sandu, Ion Sandu