TITLE:
Effect of Hyperglycemia on Erythrocyte Carbonic Anhydrase and Lactic Acid in Type II Diabetic Subjects
AUTHORS:
Salihu Ismail Ibrahim, Ameh Danladi Amodu, Atawodi Sunday Ene-Ojo, Umar Alhaji Ismaila, Mohammed Fakhruddeen
KEYWORDS:
Carbonic Anhydrase, Lactic Acid, Glycated Hemoglobin, Type II Diabetes
JOURNAL NAME:
Journal of Diabetes Mellitus,
Vol.6 No.2,
May
6,
2016
ABSTRACT: Background: Carbonic anhydrase (CA) is a ubiquitous enzyme catalyzing the
reversible hydration of CO2 to and H+. CA plays a crucial role in
CO2 transport, acid-base balance, and in linking local acidosis to O2 unloading from hemoglobin and also facilitates lactate shuttling across the
monocarboxylate transporters (MCT). The study aimed to investigate the
influence of hyperglycemia on erythrocyte carbonic anhydrase activity and
lactic acid in type II diabetic patients. Method: Red blood cell carbonic
anhydrase activity was determined in washed lysed-hemolysate by the action of
the enzyme on the substrate p-nitrophenyl acetate. The absorbance of released
p-nitrophenol was recorded at 345 nm. Glycated Hemoglobin was determined by ion
exchange method (Spectrum Diagnostic Kit). Blood glucose, lactate, cholesterol
and triglyceride were determined using Accutrend GCT meter (Roche, Germany)
with cobias®test strips. Results: The present study showed that
hyperglycemia significantly (p