TITLE:
DC Maturation: A Brief Comparison between Three Different Processes
AUTHORS:
Maria Toloudi, Ioannis Papasotiriou
KEYWORDS:
DC Maturation, TNFa, IL-4, IFNa
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.6 No.10,
September
18,
2015
ABSTRACT: Dendritic cell (DC) maturation approved to be a pivotal process for
initiating immunity. Many protocols were established in order the isolated
peripheral blood mononuclear cells (PBMCs) from healthy donors to mature into
dendritic cells (DCs). The purpose of this study was to present an effective
and reliable DC maturation procedure by comparing three different protocols
(Interleukin-4/Tumor Necrosis Factor-appha (IL-4/TNFa) DC protocol, Interferon
alpha (IFNa) DC protocol and FAST DC protocol). Whole blood was collected from
six healthy donors and PBMCs were isolated by Ficoll gradient centrifugation.
The counted cells were incubated with the addition of three different cocktails
of supplements for appropriate time period. The final mature DC population was
examined either by its phenotypic characteristics under light microscope or by
measuring the expression of antigen presenting molecules such as CD80 and CD86
by flow cytometry. It was found that the mature DCs, generated from the
IL-4/TNFa DC protocol, expressed higher levels of CD80 and CD86. Furthermore,
they sharply exhibited their phenotypic hallmarks.