TITLE:
A Preliminary Study on the Preparation of Monoclonal Antibody with Umbilical Cord Blood Cell
AUTHORS:
Dongmei Su, Shuren Zhang, Shuzhi Zhang, Ying Gong, Shaohui Zhu, Liyan Zhang, Youjuan Wang, Changqing Yue, Xiaoping Liu
KEYWORDS:
Umbilical Cord Blood Cells, Monoclonal Antibody, Rubella Virus
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.3 No.3,
March
18,
2015
ABSTRACT:
Objective: We conducted a preliminary study
on the feasibility of preparing monoclonal antibody by umbilical cord blood
cells systems. Methods: Collected umbilical cord blood cells and stimulated by
Rubella virus, then incubated them and collected cell supernatant. By using
Caprylic acid- saturated Ammonium sulfate method, monoclonal antibody was purified,
and IgG subtype identification was conducted by their subtype classification
kit of sigma. In traditional way, the preparation of monoclonal antibody cannot
do without BABL/C mice and SP2/0 hybridoma cells. Consider this kinds of monoclonal
antibody as a positive control, the reactivity and specificity of monoclonal
antibodies were identified by Dot-ELISA. Results: By ELISA, we obtained four
strains of positive umbilical cord blood cells. After
subculture, cryopreservation and resuscitation in vitro, three of them were
confirmed to secrete monoclonal antibody against rubella virus stably. The
result of monoclonal antibody subtype classification showed that, both IgG1 and
IgG2 types were detected. However, the quantity of monoclonal antibody prepared by umbilical cord blood cells was less than that from traditional
method. Conclusions: The method of prepare monoclonal antibody using umbilical
cord blood cells is feasible.