TITLE:
Performance Characteristics of ELISA to Detect Bovine Viral Diarrhea Virus (BVDV) Antibodies Using Colostrum
AUTHORS:
Caitlin J. Jenvey, Andrew M. Weir, Michael P. Reichel, Peter D. Cockcroft
KEYWORDS:
BVDV, Colostrum, ELISA, Sensitivity, Specificity
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.5 No.2,
February
13,
2015
ABSTRACT: Colostrum contains substantially higher concentrations of immunoglobulins (Igs) when compared with serum or milk, which may improve the diagnostic sensitivity of an antibody ELISA when using colostrum. In this study, BVD was used as a model to identify the performance characteristics of colostrum and to assess the potential for increased ELISA sensitivity when compared with serum. Blood and colostrum samples were collected from cows within two dairy cattle herds: a previously infected and BVD-vaccinated Holstein-Friesian (positive herd) herd, and a bulk-tank milk antibody negative (negative herd) Jersey herd. All samples were tested using a commercial BVDV antibody ELISA. Median sample-to-positive (S/P) colostrum ratios were significantly higher than their respective serum counterparts, and positive herd S/P ratios were significantly higher than the respective negative herd values (P