TITLE:
Thin Layer Chromatography and Chemometric Studies of Selected Potentilla Species
AUTHORS:
Ryszard Świeboda, Agnieszka Jóźwiak, Grzegorz Jóźwiak, Monika Waksmundzka-Hajnos
KEYWORDS:
Pomolic Acid, Ursolic Acid, Euscaphic Acid, Tormentic Acid, Oleanolic Acid, Triterpene Acids, TLC Fingerprint, Potentilla
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.5 No.16,
November
25,
2014
ABSTRACT: Herbal medicinal
products have a long-standing therapeutic record. To ensure the quality of
herbal products specific identification tests which allow experts to
discriminate related species and/or potential adulterants/substitutes are
required. The purpose of the research was to recommend an original and simple
method for the separation of closely related five triterpenic acids (ursolic,
oleanolic, tormentic, euscaphic, pomolic acid) and its application to
chemotaxonomy studies. 17 standard samples of Potentilla species and 3 test samples were chromatographed (with or
without prechromatographic derivatization) on silica gel plates using the
mobile phase: chloroform-diethyl ether-methanol-formic acid (30:10:1:0.2 v/v/v/v);
they were subsequently derivatized, and visualized in UV 366 nm light. After images
received pre-treatment (montaging stack, removal of noise, background subtract,
horizontal equalization, two types of warping) exploratory analysis of the
investigated Potentilla species
fingerprints was processed. The method without prechromatographic
derivatization was used to create differential fingerprints. Good results were
obtained by the application of r-project iterative algorithm for subtract
background and our simple algorithm for warping to image processing of TLC
chromatograms for the first time.