TITLE:
Analysis of Diagnostic Efficiency of Excretory-Secretory Antigens for Trichinella spiralis and Trichinella nativa in ELISA
AUTHORS:
Irina Odoevskaya, Sergey Movsesyan, Mikhail Voronin, Nataya Chiljuta
KEYWORDS:
ELISA, Trichinella nativa, Diagnostic Efficiency, Laboratory Infection, Wistar Rats, Blood Sera, Excretory-Secretory Antigen
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.4 No.9,
September
4,
2014
ABSTRACT: The comparative
studies of diagnostic efficiency of excretory-secretory antigens of Trichinella
spiralis and Trichinella nativa were performed using blood sera of
rats from Wistar line experimentally infected with Arctic trichinellae. For
animal infection and antigen preparation Trichinella from muscles of
wild carnivorous mammals from Arctic regions of Russia were used. When antigen
from T. nativa larvae was used to analyze titers of sera of rats
experimentally infected with Arctic Trichinella, a significant increase
in efficacy ofELISAwas detected. E.g.,
sera of rats infected with trichinellae from ringed seals retained in ELISA
with T. nativa antigen values higher than diagnostic level at titers of
1:6400 - 1:12800, while titer of those same sera when using T. spiralis antigen was no higher than 1:200 - 1:400.