TITLE:
Responses of Marsilea minuta L. to Cadmium Stress and Assessment of Some Oxidative Biomarkers
AUTHORS:
Kingsuk Das, Chiranjib Mandal, Nirmalya Ghosh, Narottam Dey, Malay Kumar Adak
KEYWORDS:
Cadmium Toxicity, Marsilea minuta, Bioindicators
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.10,
May
12,
2014
ABSTRACT:
In a hydroponic
based experiment, the Cd toxicity is monitored with some cellular responses of Marsilea plant. Initially, plants were
grown under varying concentrations (0, 50, 100 and 200 μM of Cd) of cadmium (Cd)
with supplementation of 2 mM spermidine (Spd). The oxidative stress developed
by Cd overaccumulation was measured with fall in Relative Growth Rate (RGR) by
27.11% to 59.83% growth reduction over control under varying Cd treatments. The
retrieval of RGR was recovered by 1.59 folds as compared to the highest
concentration of Cd (200 μM) when plants were fed with Spd. A concomitant
degradation of chlorophyll was recorded in dose-dependant manner, however, the
retrieval was not much pronounced with Spd. On the contrary, the non-oxidant
thiol had borne more clarity with ongoing Cd concentration and appeared to be
40.51% increase maximally for GSH: GSSG at the highest concentration of Cd. Spd
has minimized the ratio by 27.4%. The recovery of osmotic turgidity was indexed
with a sharp rise in glycine betaine by 3.86 folds maximum at the highest
concentration of Cd over control which declined by 30.9% with Spd. Another
cellular response of treated plants was more evident from their isozymic
profiles with regard to superoxide dismutase (SOD), catalase (CAT), guaiacol
peroxidase (GPX). The intensity of protein expression was significantly
variable but not in band numbers as evident from Cd treated plants. In vitro enzyme assay of catalase showed
as declining trend within the limit of 33.13% to 43.22% which was reported by
1.45 folds when Spd was applied. Therefore, from the present study, the
cellular responses of Marsilea plant
which showed compatibility for their expression with Cd toxicity could be
hypothesized as a case of bioindication.