TITLE:
Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions
AUTHORS:
Mambo Moyo
KEYWORDS:
Horseradish Peroxidase; Maize Tassel; Multiwalled Carbon Nanotubes; Metal Biosensor; Zn2+
JOURNAL NAME:
Open Journal of Applied Biosensor,
Vol.3 No.1,
February
17,
2014
ABSTRACT: Maize tassel-multiwalled carbon nanotube (MT-MWCNT) composite has been used as a matrix for physical adsorption of horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The HRP/MT-MWCNT biosensor was applied for the detection of Zn2+ in aqueous solution. The biosensor designed was able to determine Zn2+ in the range of 0.35 - 12 mg/L with a detection limit of 7.5 μg/L. The inhibition was found to be reversible and uncompetitive when data were modeled using the Dixon and Cornish-Bowden plots. The biosensor was found to have good repeatability, reproducibility and high selectivity. The developed biosensor can be used to detect other HRP inhibiting trace metal ions.