TITLE:
A method to synthesize cDNA constructs by homology based recombination cloning
AUTHORS:
Neetu Verma, Pradeep Kumar Burma
KEYWORDS:
Homology Based Recombination Cloning; cDNA Assembly
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.4 No.1,
January
3,
2014
ABSTRACT:
We introduce a homology-based
recombination approach for generating a cDNA construct. This method depends on amplifying
several exon fragments and their fusions by the homology-based recombination. This
method provides a way to generate the cDNA sequence of any gene without any
need for its mRNA. The paper describes the
strategy by assembling cDNA of the MYB1
and MYB2 genes of Arabidopsis thaliana.