TITLE:
In Vitro Studies of NIPAAM-MAA-VP Copolymer-Coated Magnetic Nanoparticles for Controlled Anticancer Drug Release*
AUTHORS:
Soodabeh Davaran, Abolfazl Akbarzadeh, Kazem Nejati-Koshki, Somayeh Alimohammadi, Mahmoud Farajpour Ghamari, Mahsa Mahmoudi Soghrati, Akbar Rezaei, Amir Ahmad Khandaghi
KEYWORDS:
Magnetic Nanoparticles; Thermosensitive Copolymer; Doxorubicin; P(NIPAAM-MAA-VP) Copolymer
JOURNAL NAME:
Journal of Encapsulation and Adsorption Sciences,
Vol.3 No.4,
December
20,
2013
ABSTRACT:
Thermosensetive poly(N-isopropylacrylamide)-based
magnetic nanoparticles were synthesized by free radical polymerization
of N-isopropylacrylamide (NIPPAMs), methacrylic acid (MAA), and vinyl
pyrrolidone (VP) in the presence of methylene-bis-acrylamide as cross linking
agent. The Fe3O4 magnetic nanoparticls were prepared by
chemical precipitation of Fe salts in the ratio of 1:2 under alkaline and inert
condition. Thermosensitive crosslinked P (NI-PAAM-MAA-VP)
copolymers were characterized by FT-IR and H-NMR. The pH and
thermosensitive copolymer was used for preparation of drug loaded magnetic
nanoparticles, and doxorubicin (DOX) was used as a typical anticancer drug. The amount
of the loaded drug and drug release amount were determined by UV measurements. Scanning
electron microscopy (SEM) and lower critical solution temperature
(LCST) were used to determine the particle surface morphology and the phase
transition temperature of the nanoparticles respectively. The release
behavior of DOX at pH = 7.4 and 37°C was studied. The result indicated that
this thermosensetive magnetic nanoparticle has a high drug loading
capacity and favorable linear release property for DOX without initial burst
release. Thus this system is promising for the application in targeted smart
anticancer drug delivery.