TITLE:
In Silicon Cloning and Bioinformatics Analysis of the Raphanus Sativus WUS Gene
AUTHORS:
Ma Guang
KEYWORDS:
Raphanus sativus; WUS; Bioninformatics; Stem Cell
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
December
20,
2013
ABSTRACT:
The complete coding sequence ofRaphanus sativusWUS gene was obtained by usingArabidopsis thalianagene se-quence
(NM_127349) as a probe in silico cloning. Then the hydrophilicity, secondary
structure and advanced structure of WUS protein inRaphanus sativusare analyzed by using bioinformatics methods. The
results show that the cDNA was 1298 bp, with no intron, contains an open
reading frame of 936bp, encoding 312aa protein. The protein coded byRaphanus sativusgene showed 74%
similarity toArabidopsis thaliana.