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has been cited by the following article:

• TITLE: Improved Micropropagation in Hybanthus enneaspermus L. Muell

  AUTHORS: Gandhi Premkumar, Neelakantan Arumugam, Sankaralingam Muthuramkumar, Govidaraju Varatharaju, Kaniappan Rajarathinam

  KEYWORDS: Hybanthus enneaspermus; Multiple Shoots; Regeneration; Morphology

  JOURNAL NAME: American Journal of Plant Sciences, Vol.4 No.6, June 10, 2013

  ABSTRACT: Multiple shoots were induced on the cut ends of the leaf of Hybanthus enneaspermus on MS medium fortified with different concentration of cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the multiple shoots induction, different types of auxins were used. IAA induced a maximum of 52.3 shoots per explants in combination with KI + BAP. The regenerated shoots show two different morphological leaves. Shoots are 100% rooted on half-strength MS medium supplemented with 2.46 μM·l-1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization and they appeared morphologically normal.